Erythrocyte Inclusions - Wright Stained Smears

Marjorie A. Spahn, MT (ASCP); Marybeth Fannon, MA, MT (ASCP)

This course describes and richly illustrates morphology of erythrocyte inclusions, including reticulocytes (polychromasia), basophilic stippling, Cabot rings, Pappenheimer bodies, siderotic granules, and Howell-Jolly bodies. Good for CE, exam review and cross training.

Continuing Education (CE) Credits

P.A.C.E. Contact Hours: 2.5 hour(s)
Florida CE: General (Hematology): 2 hour(s)

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Erythrocyte Inclusions - Wright Stained Smears Objectives

Define the term ERYTHROCYTE INCLUSIONS.
Explain why it is important to identify ERYTHROCYTE INCLUSIONS.
Discuss: RETICULOCYTES (POLYCHROMASIA), BASOPHILIC STIPPLING, and CABOT RINGS with respect to: stain used, appearance, composition, and associated physiological defects.
Discuss: PAPPENHEIMER BODIES, SIDEROTIC GRANULES, and HOWELL-JOLLY BODIES with respect to: stain used, appearance, composition, and associated physiological defects.
Discuss the use of Perl's PRUSSIAN BLUE IRON stain to confirm the presence of IRON containing GRANULES.
Identify red cell INCLUSIONS with 80% accuracy when given 2 x 2 KODACHROME slides or PERIPHERAL BLOOD SMEARS.

Erythrocyte Inclusions - Wright Stained Smears Outline

Introduction to ERYTHROCYTE INCLUSIONS

What is ERYTHROCYTE INCLUSION?
More on ERYTHROCYTE INCLUSIONS
The presence of ERYTHROCYTE INCLUSIONS may indicate the presence of DISEASE.
Each INCLUSION is specific for a disorder.

Characteristics of RETICULOCYTES (POLYCHROMASIA)

RETICULOCYTES
More on RETICULOCYTES
ERYTHROCYTE Production
Stress RETICULOCYTES
Normal Red Cells
POLYCHROMATOPHILIC Cells
Another POLYCHROMATOPHILIC Cell
Stress RETICULOCYTE
Another Stress RETICULOCYTE
POLYCHROMATOPHILIC Cell Absence
Which of the following statements characterize a RETICULOCYTE? (Choose ALL of the correct answers)
On a WRIGHT's-stained smear, the blue/gray appearing NON-NUCLEATED red cells are called:
The presence of shift RETICULOCYTES is an indication that red cells are leaving the MARROW earlier than normal.
This slide contains:
This slide contains:
This slide contains:
This slide contains:

Characteristics of BASOPHILIC STIPPLING

What is BASOPHILIC STIPPLING?
More on BASOPHILIC STIPPLING
Fine and Coarse BASOPHILIC STIPPLING
BASOPHILIC STIPPLING
Fine BASOPHILIC STIPPLING
POLYCHROMATOPHILIC Cell
Coarse BASOPHILIC STIPPLING
Another Coarse BASOPHILIC STIPPLING
Two POLYCHROMATOPHILIC Cells
What is the composition of BASOPHILIC STIPPLING?
What kind of BONE MARROW activity is associated with fine BASOPHILIC STIPPLING?
Disorders in which coarse BASOPHILIC STIPPLING can be seen are: (Choose ALL of the correct answers)
The cells in the slide are normal cells.
The cells in this slide show:
The cells in this slide show:
The cells in this slide show POLYCHROMASIA.

Characteristics of HOWELL-JOLLY BODIES

What are HOWELL-JOLLY BODIES?
More on HOWELL-JOLLY BODIES
HOWELL-JOLLY BODY
Another HOWELL-JOLLY BODY
HOWELL-JOLLY BODIES in CYTOPLASM
HOWELL-JOLLY BODY in a Nucleated Red Cell
What is the composition of HOWELL-JOLLY BODIES?
If present, how many HOWELL-JOLLY BODIES are usually seen within each ERYTHROCYTE?
In which of the following conditions would you expect to find HOWELL-JOLLY BODIES?
What ABNORMALITY is present in this slide?
This slide shows BASOPHILIC STIPPLING.
What ABNORMALITY is present in this slide?
What ABNORMALITIES can be seen in this slide?
There are no ABNORMALITIES in this slide.
What ABNORMALITIES are present in this slide?
What ABNORMALITY is present in this slide?

Characteristics of PAPPENHEIMER BODIES and SIDEROTIC GRANULES

What are PAPPENHEIMER BODIES?
More on PAPPENHEIMER BODIES
HOWELL-JOLLY BODY and PAPPENHEIMER BODIES
Three PAPPENHEIMER BODIES
BASOPHILIC STIPPLING contrasted with PAPPENHEIMER BODIES
HOWELL-JOLLY BODIES contrasted with PAPPENHEIMER BODIES.
Another HOWELL-JOLLY BODY PAPPENHEIMER BODY contrast.
Three Way Comparison
SIDEROTIC GRANULES
SIDEROCYTES and Ring SIDEROBLASTS.
SIDEROCYTES
SIDEROBLAST
Another SIDEROBLAST
Ring SIDEROBLAST
SIDEROTIC GRANULES
Ring SIDEROBLAST
Which of the following statements best describes PAPPENHEIMER BODIES?
In a WRIGHT's-stained smear, PAPPENHEIMER BODIES must be differentiated from BASOPHILIC STIPPLING and HOWELL-JOLLY BODIES. Pick the statement which best describes each of the following.
Match the following terms with the correct definition.
What is the reason for using Perl's PRUSSIAN BLUE stain?
In which of the following conditions are PAPPENHEIMER BODIES and SIDEROCYTES seen on the peripheral smear?
Which of the following is considered a PATHOLOGICAL finding?
The red cell INCLUSIONS present in this field are known as ______ JOLLY bodies.
This slide shows fine BASOPHILIC STIPPLING.
This smear shows:
The smear several HOWELL-JOLLY BODIES as well as several _____________ bodies.
This smear shows PAPPENHEIMER BODIES.
This PRUSSIAN BLUE stained smear shows:
The INCLUSION seen in the red cell just above center in this field is a ___________ BODY.
The red cell in the center of the field shows _________ ___________.
This smear shows several POLYCHROMATOPHILIC red cells.
This slide contains a SIDEROBLAST.
The red cell in the center of the field contains a:

Characteristics of CABOT RINGS

What are CABOT RINGS?
CABOT RINGS
More CABOT RINGS
CABOT RING Impersonation
Which of the following statements best describes the appearance of CABOT RINGS?
Identify the conditions in which CABOT RINGS are occasionally seen.

Erythrocyte Inclusions - Wright Stained Smears Keywords

Click on a term below to see its use in this Erythrocyte Inclusions - Wright Stained Smears course and other available MediaLab courses.

basophilic stippling (40 occurances)
pappenheimer bodies (27 occurances)
erythrocyte (21 occurances)
howell-jolly bodies (21 occurances)
iron (15 occurances)
inclusion (14 occurances)
granules (14 occurances)
polychromasia (14 occurances)
reticulocyte (14 occurances)
prussian blue (13 occurances)
sideroblast (13 occurances)
cabot ring (13 occurances)
howell-jolly body (13 occurances)
siderocyte (12 occurances)
polychromatophilic (12 occurances)
wright (9 occurances)
nucleus (9 occurances)
anemia (9 occurances)
siderotic granules (8 occurances)
abnormality (7 occurances)
cytoplasm (7 occurances)
erythrocyte inclusion (6 occurances)
bone marrow (6 occurances)
nuclear (6 occurances)
megaloblastic (6 occurances)
hemoglobin (6 occurances)
jolly (6 occurances)
immature (5 occurances)
pathological (5 occurances)
splenectomy (5 occurances)
spindle (4 occurances)
ribosomes (4 occurances)
wrights-stained (4 occurances)
mitotic (4 occurances)
body (4 occurances)
dna (3 occurances)
deficiency (3 occurances)
abnormalities (3 occurances)
blood (3 occurances)
methylene (3 occurances)
organelles (3 occurances)
granular (3 occurances)
hemolytic (3 occurances)
sickle (3 occurances)
pyknotic (3 occurances)
pappenheimer body (3 occurances)
polycythemia (2 occurances)
prussion (2 occurances)
pappenheimer (2 occurances)
siderotic (2 occurances)
stippling (2 occurances)
wright-giemsa (2 occurances)
thalassemia (2 occurances)
iron-containing (2 occurances)
leukemia (2 occurances)
myeloma (2 occurances)
abnormal (2 occurances)
cabot (2 occurances)
chromosomes (2 occurances)
erythropoiesis (2 occurances)
feulgen (2 occurances)